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Yes, that お before 前 (in front) is the same keigo honorific prefix we've seen before, but nowadays it has a sarcastic nuisance that makes it slightly disrespectful:
https://jisho.org/word/%E3%81%8A%E5%89%8D
If your little brother is misbehaving, you can just say "お前...", and that alone should suffice, you can omit the rest of the sentence.
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